Deficiency of nitric oxide in polycation-induced airway hyperreactivity

摘要

Using a perfused guinea-pig tracheal tube preparation, we investigated the role of endogenous nitric oxide (NO) in polycation-induced airway hyperreactivity (AHR) to methacholine. Intraluminal (IL) administration of the NO synthase inhibitor N~ω-nitro-l-arginine methyl ester (L-NAME; 100μM) caused a 1.8 fold increase in the maximal contractile response (E_max) to IL methacholine compared to control, without an effect on the pEC_50 (-log_10 EC_50).