Effect of steady and oscillatory shear stress on F-actin content and distribution in neutrophils

摘要

We investigated neutrophil activation, specifically F-actin content and distribution, in situations mimicking the in vivo environment using steady and oscillatory shear. Under low steady shear (< 150 s~(-1)) F-actin levels were decreased for both treated (n-formyl-L-methioryl-L-leucyl-L-phenylalanine (fMLP)) and untreated neutrophils. The F-actin content increased with a change to higher steady shear levels. Neutrophils show the same behavior of decreased F-actin content for oscillatory shear (26.7 s~(-1)) as they did for steady shear. In both situations, the low shear levels caused a decrease in F-actin content. However, as the magnitude of the shear rate increased, cells showed a reversal to increasing F-actin content. Shear caused a decrease in F-actin in the cell cortex for both control and fMLP treated cells. Ctyochalasin B (CB), a common F-actin assembly blocker, significantly decreased F-actin content. The results indicate that neutrophils regulate their actin network based on the level and type of shear stress they encounter in the bloodstream.