Production of thermostable β-amylase and pullulanase by Clostridium thermosulfurogenes SV2 in solid-state fermentation; Screening of nutrients using Plackett-Burman design

摘要

Screening of fifteen nutrients belonging to four categories, viz., carbon, nitrogen, salt and complex organic sources was carried out using Plackett-Burman design for the production of thermostable β-amylase and pullulanase by Clostridium thermosulfurogenes SV2 in solid-state fer- mentation (SSF). This design involves screening of up to 'n-1' variables in just 'n' number of experiments. Re- gression co-efficients and t-values were calculated by subjecting the experimental data to statistical analysis.