Electrochemical biofilm control: mechanism of action

摘要

Although it has been previously demonstrated that an electrical current can be used to control biofilm growth on metal surfaces, the literature results are conflicting and there is no accepted mechanism of action. One of the suggested mechanisms is the production of hydrogen peroxide (H_2O_2) on metal surfaces. However, there are literature studies in which H_2O_2 could not be detected in the bulk solution. This is most likely because H_2O_2 was produced at a low concentration near the surface and could not be detected in the bulk solution. The goals of this research were (1) to develop a well-controlled system to explain the mechanism of action of the bioelectrochemical effect on 316L stainless steel (SS) surfaces and (2) to test whether the produced H_2O_2 can reduce cell growth on metal surfaces. It was found that H_2O_2 was produced near 316L SS surfaces when a negative potential was applied. The H_2O_2 concentration increased towards the surface, while the dissolved oxygen decreased when the SS surface was polarized to - 600 mV_(Ag/AgCl). When polarized and non-polarized surfaces with identical Pseudomonas aeruginosa PAO1 biofilms were continuously fed with air-saturated growth medium, the polarized surfaces showed minimal biofilm growth while there was significant biofilm growth on the non-polarized surfaces. Although there was no detectable H_2O_2 in the bulk solution, it was found that the surface concentration of H_2O2 was able to prevent biofilm growth.