首页> 外文期刊>Biochimie >In maize, two distinct ribulose 1,5-bisphosphate carboxylase/oxygenase activase transcripts have different day/night patterns of expression
【24h】

In maize, two distinct ribulose 1,5-bisphosphate carboxylase/oxygenase activase transcripts have different day/night patterns of expression

机译:在玉米中,两种不同的核糖1,5-双磷酸羧化酶/加氧酶激活酶转录物的昼夜表达方式不同

获取原文
获取原文并翻译 | 示例
       

摘要

Several cDNAs encoding ribulose-1,5-bisphosphate carboxylase/oxygenase activase (Rubisco activase, RCA) were isolated from a maize (Zea mays L.) leaf cDNA library. Although all the cDNAs encoded the same polypeptide, the RCA 0 isoform, they showed two different downstream-like elements (DST-like) at their 3' untranslated regions (UTRs). The Zmrca1 cDNAs had the subdomain I, and II and the Zmrca2 cDNAs, besides these subdomains, showed two repeats of the subdomain III. The presence of at least two different rca genes in the maize genome was demonstrated by Southern, and by PCR analysis using primers specific for the two cDNAs. Northern analysis with probes specific! for each gene showed that the Zmrca2 was expressed as a 1.8 kb transcript, the Zmrca1 corresponded to a 1.4 kb transcript, and a 1 kb band was a stable degradation product of one or both transcripts. Although both mRNAs showed cyclic variations during a day/night period, with their highest levels before dawn, the Zmrca2 transcript showed stronger changes than the Zmrca1 transcript, presenting a twofold larger highest to lowest RNA accumulation ratio than the Zmrca1 transcript, implying that they have different turnover rates. Our results suggest that post-transcriptional mechanisms, mediated by the DST-like element might be involved in the circadian expression of the maize rca transcripts. (C) 2004 Elsevier SAS. All rights reserved.
机译:从玉米(Zea mays L.)叶cDNA文库中分离出几个编码核糖-1,5-双磷酸羧化酶/加氧酶激活酶(Rubisco activase,RCA)的cDNA。尽管所有cDNA都编码相同的多肽,即RCA 0亚型,但它们在其3'非翻译区(UTR)上显示了两个不同的下游样元件(DST-like)。 Zmrca1 cDNA具有子结构域I和II,而Zmrca2 cDNA除了这些子结构域之外,还具有子结构域III的两个重复。玉米基因组中至少有两个不同的rca基因的存在已通过Southern鉴定,并通过使用对这两个cDNA特异的引物进行PCR分析得以证实。用探针进行北方分析!对于每个基因,Zmrca2均以1.8 kb的转录本表达,Zmrca1对应于1.4 kb的转录本,而1 kb的条带则是一个或两个转录本的稳定降解产物。尽管这两种mRNA在白天/晚上都表现出周期性变化,但黎明前的水平最高,但Zmrca2转录本的变化要强于Zmrca1转录本,其最高至最低RNA积累比是Zmrca1转录本的两倍,这表明它们具有不同的流失率。我们的结果表明,由DST样元件介导的转录后机制可能与玉米rca转录物的昼夜节律表达有关。 (C)2004 Elsevier SAS。版权所有。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号