Cell culture media are potent antioxidants that interfere during LDL oxidation experiments

摘要

In vitro cell-induced low-density lipoprotein (LDL) oxidation is a model frequently used for studies on antioxidant compounds which may be potentially antiatherogens. Using Cu2+ or the free radical generator 2,2'-azobis-[2-amidinopropane] dihydrochloride (AAPH) to oxidize human LDL. we showed that the cell culture media Ham's F10 and RPM1 are potent antioxidants which reduce LDL-protective effect of various thyroid compounds. The culture media interfered with the compounds depending on their mechanism of action, and RPM1 had the Greatest antioxidant effect. completely hiding, antioxidant efficiency of the compounds whatever the prooxidant agent was. We suggest some recommendations for study of antioxidant compounds using cell-induced LDL oxidation models. (C) 2004 Elsevier SAS. All rights reserved.