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Purification of a Class B1 platelet aggregation inhibitor phospholipase A(2) from Indian cobra (Naja naja) venom

机译:从印度眼镜蛇(眼镜蛇)毒液中纯化B1类血小板聚集抑制剂磷脂酶A(2)

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A platelet aggregation inhibitor phospholipase A, (NND-IV-PLA(2)) was isolated from Naja naja (Eastern India) venom by a combination of cation and anion exchange chromatography. NND-IV-PLA(2) is the most catalytically active enzyme isolated from the Indian cobra venom. The acidic PLA(2) profile of Eastern regional Indian cobra venom is distinctly different from that of the western regional venom. However the acidic PLA(2)s from both the regions follow the pattern of increasing catalytic activity with increase in acidic nature of the PLA(2) isoform. NND-IV-PLA(2) is a Class B1 platelet aggregation inhibitor and inhibits platelet aggregation induced by ADP, collagen and epinephrine. Modification of active site histidine abolishes both catalytic activity and platelet aggregation inhibition activities while aristolochic acid, a phospholipase A(2) inhibitor has only partial effect on the two activities
机译:通过结合阳离子交换和阴离子交换色谱法从眼镜蛇(印度东部)毒液中分离出血小板聚集抑制剂磷脂酶A(NND-IV-PLA(2))。 NND-IV-PLA(2)是分离自印度眼镜蛇毒液的最具催化活性的酶。东部地区印度眼镜蛇毒的酸性PLA(2)谱与西部地区毒的酸性PLA明显不同。但是,来自两个区域的酸性PLA(2)都遵循随着PLA(2)同工型的酸性性质增加而增加的催化活性的模式。 NND-IV-PLA(2)是B1类血小板聚集抑制剂,可抑制ADP,胶原蛋白和肾上腺素诱导的血小板聚集。活性位点组氨酸的修饰消除了催化活性和血小板聚集抑制活性,而马兜铃酸,一种磷脂酶A(2)抑制剂仅对这两种活性有部分影响

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