Bifunctional structure of two adenylyl cyclases from the myxobacterium Stigmatella aurantiaca

摘要

Two adenylyl cyclase genes (cyaA and cyaB) from the myxobacterium Stigmatella aurantiaca were cloned by complementation of Escherichia coli mutants defective in the cya gene. cyaA codes for a protein of 424 amino amino acid residues (AC1), while cyaB encodes a protein of 352 residues (AC2). Both cyclases are sensitive to adenosine: camp production was strongly inhibited in E coli cells and cell extracts expressing these genes.