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Protein translocation functions of Escherichia coli SecY: in vitro characterization of cold-sensitive secY mutants

机译:大肠杆菌SecY的蛋白质易位功能:冷敏感secY突变体的体外表征

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摘要

Protein translocation across the plasma membrane of E coli is facilitated by Sec factors, including the membrane-embedded Sec YEG subunit and the SecA ATPase. Although there is complete agreement that SecA is essential for protein translocation, some publications question the essentialness of SecY. We previously isolated a number of cold-sensitive mutants of secY and characterized their in vivo phenotypes.
机译:Sec因子,包括膜包埋的Sec YEG亚基和SecA ATPase,促进了蛋白质在大肠杆菌质膜上的转运。尽管完全一致认为SecA对于蛋白质易位至关重要,但一些出版物质疑SecY的必要性。我们之前分离了许多secY的冷敏感突变体,并对其体内表型进行了表征。

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