Chlamydomonas U2, U4 and U6 snRNAs. An evolutionary conserved putative third interaction between U4 and U6 snRNAs which has a counterpart in the U4_atac-U6_atac snRNA duplex

摘要

The spliceosomal UsnRNAs U2, U4 and U6 from the green alga Chlamydomonas reinhardtii (Cre) were sequenced using a combination of RNA and cDNA sequencing methods and were compared to other sequenced UsnRNAs. The lengths of Cre U6 and Cre U2 RNAs are similar to those of their higher plant equivalents. Cre U4 RNA is shorter (139 nt) The its counterpart from higher plants (150-154) nt), and contains stem IV and loop D which are absent, with the exception of the Tetrahymena U4 RNA, from the U4RNAs of other unicellular organisms studied to date.