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GTP-dependent modification of a 21-kDa substrate with NAD~+ in bobine brain soluble fraction is not ADP-ribosylation of small G-protein but tailing of tRNA

机译:大脑脑可溶部分中NAD〜+对21 kDa底物的GTP依赖性修饰不是小G蛋白的ADP核糖基化,而是tRNA的拖尾

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摘要

Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-~32P] NAD~+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C_3 substrate in size, was labeled even without C_3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP inhibited the GTP-induced labeling of 21-Kda substrate.
机译:当牛脑可溶级分与[腺苷酸-〜32P] NAD〜+在GTP存在下孵育时,观察到21 kDa物质的标记。即使没有C_3外切酶,也标记了21-kDa底物,其大小略小于C_3底物。 GTP可以被ATP以外的核苷三磷酸替代,而ATP抑制了GTP诱导的21-Kda底物的标记。

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