Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs

摘要

A method to synthesise region-labelled proteins for structural studies with NMR is suggested. The technique is based on in vitro translation of matrix-coupled mRNAs. Translation starts with unlabelled amino acids from the initiation codon of the mRNA and continues to the beginning of the region of interest. Here, the ribosomes pause while the tRNAs charged with unlabelled amino acids are replaced with rRNAs charged with isotope-labelled amino acids.