Artefactual cleavage of E coli H-NS by OmpT

摘要

In the bacterium Escherichia coli, H-NS (H1, H1a) is a heat-stalbel protein with a molecular mass of 15.5 kDa involved in nucleoid organization and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of prep- arations of everted inner membrane vesicles, heat-stable cleavage products of approximately 10 kDa of H_NS are formed in vitro from newly synthesised, radio-labelled H-Ns and from purified H-NS.