S6 ribosomal protein phosphorylation and translation of stored mRNA in maize

摘要

This article focuses on the effect that S6 ribosomal protein phosphorylation might have in regulating mRNA translation. Maize axes of either 4 or 14 h of germination were pulse-labelled for 1 h with [~32P]-orthophosphate. Analysis of their ribosomal proteins by gel electrophoresis and autoradiography showed distinctive levels of S6 ribosomal protein phosphorylation for both ribosomal sets. Axes at these two stages of germination were treated with α-amanitin to ensure transcription inhibition and pulse-labelling with [~35S]-methionine.