Effect of N0terminal deletions on the activity of pokeweed antiviral protein expressed in E.coli

摘要

Pokeweed antiviral protein(PAP) from Phytolacca americana is a highly specific N0glycosidase removing adenine residues(A~4324 in 28S rRNA and A~2660in 23S rRNA) from intact ribosomes of both eukaryotes and prokaryotes. Due to the ribosome impairing activity the gene coding for mature PAP has not been expressed so far in bacteria whereas the full-length gene(coding for the mature 262 amino acids plus two signal peptides of 22 and 29 amino acids at both N- and C-termini, respectively) has been expressed in Escherichia coli.