A poflavodoxin: Structure, stability, and FMN binding

摘要

Flavodoxins are one domain α/β electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononuc1eotide(FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavodoxins; the latter contain an extra loop with unknown function. We have undertaken the study of the stability and folding of the apoflavodoxin from Anabaena(a long flavodoxin) and the analysis of the interaction between the apoflavodoxin and FMN. Our studies indicate that apoflavodoxin folds in a few seconds on a form that is competent in FMN binding.