Visualizing multiple constrictions inspheroidal escherichia coli cells

摘要

An Escherichia coli cell grows by elongation and divides in a perpendicular plane. Alternating planes of successive divisions in three dimensions can only ascertained when multiple constrictions simultaneously in large, spheroidal cells(with extended constriction process), if the division signals are enhanced. Large, spheroidal cells are obtained by a brief mecillinam treatment, and more frequent division are achieved by manipulating the rate of chromosome replication without affecting cell mass growth rate.