Cloning and characterisation of a pea mitochondrial NDPK

摘要

Here we report the cloning of a cDNA encoding the first nucleoside diphoshate kinase(NDPK)isolated from plant mitochondria. Amplification of a 317 nt product was performed by PCR, using oligonucleotide primers based on partial amino acid sequences of the pea mitochondria NDPK and other NDP isoforms. By screening of a pa cDNA library with this PCR product, a full length clone was obtained. Northern analysis revealed the presence of a 1.1 kb single transcript, with high expression in young leaves and reproductive tissues.