Identification of a ribosomal ATPase in escherichia coli cells

摘要

Eukaryotic ribosomes harbor an ATPase activity that has been shown to be essential for translation elongation in some lower fungi. Here we report the first identification of a ribosome bound ATPase, RbbA, in E.coli cells. RbbA accounts for most of the ATPase activity associated with 70S ribosomes and 30S ribosomal subunits. Both native and rEcombinant RbbA were puridied and shown to posses ribosome-dependent ATPase activities and to stimulate polyphenylalanine synthesis in vitro.