ISOLATION AND MOLECULAR CHARACTERIZATIONS OF MARINE BACTERIA ISOLATED FROM BEAGLE CHANNEL

摘要

Marine microorganisms play an important ecological role in the biological process of transformation of marine environment. Marine bacteria exhibit different hydrolytic enzymes that degrade fractions of organic matter in the sea. Marine microorganisms are the extremophiles carry out main a role in the biodegradation of the organic matter in diverse Antarctic ecosystems. Those culturing efforts have yielded new species within the divisions of γ- and α-Proteobacteria. The aims of this work were the isolation and taxonomy characterization of marine microorganisms from Antarctic region. Samples of benthonic organisms and seawater were taken from different coastal areas of the Beagle Channel (55°S; 67°W), Tierra del Fuego, Argentina. Samples were spread in LB medium and growth at 4 and 15℃. The 95 isolated colonies were characterized by 16S rDN A and gyrB PCR amplifications. Isolates were separated into groups according to their ARDRA profiles and RFLP analysis. 16S rDN A and gyrB gene were sequenced from one isolate from each group, and the phylogenetics trees were constructed. The gene sequences allowed us to determine their association with the class Proteobacteria; members of genera Pseudomonas, Pseudoalteromonas, Serratia, Halomonas, Alteromonas, Psychrobacter, Shewanella. These studies provide a new source of strains, genes, enzymes and metabolites for biotechnological applications.