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SEX IDENTIFICATION OF SLAVE SACRIFICE VICTIMS FROM QIN STATE TOMBS IN THE SPRING AND AUTUMN PERIOD OF CHINA USING ANCIENT DNA

机译:利用古代DNA鉴定中国春秋战国时期秦国墓奴隶牺牲品的性别。

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Ancient DNA (aDNA) analysis was employed to determine the sex of slave sacrifice victims from Qin State tombs during the Spring and Autumn Period of China. It is difficult to obtain sex information from fragmentary skeleton samples with the aid of skeletal morphology methods. aDNA was extracted from the dentine in selected tooth samples of sacrificial slaves using a modified traditional method, which combines together phenol/chloroform extraction, silicon dioxide adsorption and ultrafiltration concentration. Based on the sequence differences between the amelogenin homologous gene in the X and that in the Y chromosome, a pair of specific primers was designed to identify the sex of the selected samples. In the selected eight typical samples, the aDNA analytical results revealed that three were males and two were females. These findings indicate that molecular sex identification might provide more valuable information for archaeological research on the institution of slave sacrifice in the Spring and Autumn Period of China.
机译:利用古代DNA(aDNA)分析来确定中国春秋战国时期秦国墓地奴隶牺牲受害者的性别。借助于骨骼形态学方法很难从碎片的骨骼样本中获得性别信息。使用改良的传统方法从选定的牺牲奴隶的牙齿样本中的牙本质中提取aDNA,该方法将苯酚/氯仿提取,二氧化硅吸附和超滤浓缩结合在一起。根据X和Y染色体上釉原蛋白同源基因之间的序列差异,设计了一对特异性引物来鉴定所选样品的性别。在选定的八个典型样本中,aDNA分析结果显示,三个是男性,两个是女性。这些发现表明,分子性别鉴定可能为中国春秋战国时期奴隶牺牲制度的考古研究提供更多有价值的信息。

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