SQUID-Based Biosensor for Probing Ion Transporters in Cell Suspensions and Tissue

摘要

We have developed a SQUID-based technique to probe membrane-bound proteins, such as cation transporters. The method involves excitation of a biological sample with a sinusoidal electric field (0–5 V/cm, 10–300 Hz), detection, with a SQUID magnetometer, of the magnetic field produced by ac currents in the tissue or cell suspension, and calculation of the Fast Fourier Transform (FFT) of the measured response to obtain a spectrum. We find that the harmonic response of budding yeast cells (S. cerevisiae, $10^{8}$ cells/ml) is sensitive to sodium metavanadate, an inhibitor of a proton pump known as ${rm H}^{+}$-ATPase, glucose, a substrate of ${rm H}^{+}$-ATPase, and gramicidin, a membrane depolarizer. SQUID-based field and frequency dependent harmonic response measurements are also made of a live earthworm (Lumbricus terrestris). Use of the SQUID significantly reduces spurious harmonics and distortion of the excitation field by the measurement electrodes.