Local x-ray structure analysis of optically manipulated biological micro-objects

摘要

X-ray diffraction using micro- and nanofocused beams is well suited for nanostructure analysis atndifferent sites of a biological micro-object. To conduct in vitro studies without mechanical contact,nwe developed object manipulation by optical tweezers in a microfluidic cell. Here we report x-raynmicrodiffraction analysis of a micro-object optically trapped in three dimensions. We revealed thennanostructure of a single starch granule at different points and investigated local radiation damageninduced by repeated x-ray exposures at the same position, demonstrating high stability and fullncontrol of the granule orientation by multiple optical traps. © 2010 American Institute of Physics.nu0004doi:10.1063/1.3525941