Soft cluster-induced desorption and ionization of biomolecules—Influence of surface load and morphology on desorption efficiency

摘要

Neutral cluster-induced desorption and ionization of oligopeptides both from /im-thick films as well as from surfaces prepared with submonolayer surface concentration of biomolecules was investigated by means of mass spectrometry. Highest signal intensity was observed from thick films indicating efficient desorption from bulk-like material. In the submonolayer regime, the ion signal of the desorbed biomolecules was found to depend nonlinearly on the amount of substance of the wet-chemically applied biomolecules; the observation is correlated to the formation of aggregates of biomolecules on the surface.