Ultrafast chemical-free cell lysis by high speed stream collision induced by surface acoustic waves

摘要

This paper reports on a surface acoustic wave (SAW) based cell lysis device on a LiNbO_3 substrate by utilizing high speed collision of cells, which are accelerated by acoustic streaming. With varying working powers, cell lysis was achieved within 20 s and more than 95% lysis efficiency. The cell solution volume effect on SAW based lysis was also investigated and proved that it is not the main issue. With the CCK8 based viability test and verification of cell contents by electrophoresis, the efficient lysis results of our devices have been verified.