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Electroformation from patterned single-layered supported lipid bilayers for formation of giant vesicles with narrow size distribution

机译:从形成图案的单层支持脂质双层电形成薄尺寸分布的巨大囊泡

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摘要

We describe a method of producing solvent-free giant vesicles (GVs) with a narrow size distribution from patterned supported lipid bilayers (SLBs). The SLBs were prepared on a patterned SiO_2 surface by vesicle fusion, and the GVs were formed from the SLBs by electroformation. Fluorescence observation showed the formation of single-layered SLBs on circular patterns of SiO_2 with a diameter of 20 μm, the area of which corresponded to that of a unilamellar vesicle of 10 μm diameter. The electroformation from the patterned SLBs produced the GVs with a median diameter of 8.53 μm and a coefficient of variation of 10.9%.
机译:我们描述了一种从图案化的支持脂质双层(SLBs)产生具有窄尺寸分布的无溶剂大囊泡(GV)的方法。通过囊泡融合在图案化的SiO_2表面上制备SLB,并且通过电形成由SLB形成GV。荧光观察显示在直径为20μm的SiO_2圆形图案上形成单层SLB,其面积对应于直径为10μm的单层囊泡。图案化SLB的电铸产生了GV,其中位直径为8.53μm,变异系数为10.9%。

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  • 来源
    《_Applied Physics Express》 |2014年第11期|117001.1-117001.3|共3页
  • 作者单位

    Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan;

    Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan;

    Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan,Department of Environmental and Life Sciences, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan;

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