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SHORT COMMUNICATION AN ENZYMATIC TISSUE DIGESTION METHOD FOR FIBRE BIOPERSISTENCE STUDIES

机译:短距离通信一种酶解组织消解方法,用于纤维生物存在性研究

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摘要

Measurement of the biopersistence of fibres and other dusts within lung tissue is an important aspect of hazard assessment. Fibres that are persistent within lung tissue are believed to be more hazardous than less persistent fibres (Davis, 1994). The determination of lung burden in animal biopersistence studies requires that fibres are recovered without loss or damage (Warheit et al., 1991). If analysis is to be by electron microscopy, preparations must also contain a minimum of lung residue. A number of reagents have been used to recover fibres from lung tissue including sodium hypochlorite (bleach), potassium and sodium hydroxide, hydrochloric acid and hydrogen peroxide (Davis et al., 1986).
机译:肺组织内纤维和其他粉尘的生物持久性的测量是危害评估的重要方面。人们认为,在肺组织中持久存在的纤维比持久性较低的纤维更具危害性(Davis,1994)。在动物生物持久性研究中确定肺负担需要回收纤维而不会造成损失或损坏(Warheit等,1991)。如果要通过电子显微镜进行分析,则制剂还必须包含最少的肺残留物。已经使用了许多试剂从肺组织中回收纤维,包括次氯酸钠(漂白剂),氢氧化钾和氢氧化钠,盐酸和过氧化氢(Davis等,1986)。

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