PRODUCTION OF VOLATILE METABOLITES FROM STREPTOMYCES ALBIDOFLAVUS CULTIVATED ON GYPSUM BOARD AND TRYPTONE GLUCOSE EXTRACT AGAR--INFLUENCE OF TEMPERATURE, OXYGEN AND CARBON DIOXIDE LEVELS

摘要

Streptomyces albidoflavus was cultivated on tryptone glucose extract agar (TGEA) and gypsum board in culture flasks. The medium, the cultivation temperature, and the oxygen and carbon dioxide levels in the supplied air were varied multivariately. Air samples from the cultures were adsorbed on Tenax TA, analysed with the use of thermal desorption-cold trap injection gas chromatography, and identified by mass spectrometry. Alcohols, ketones, terpenes and terpene derivatives, for example geosmin, were produced on both media. On TGEA, sulphur compounds were the dominating products, dimethyl disulphide being produced in major amounts. The medium and the temperature exerted the highest influence on production, but the oxygen and carbon dioxide levels also affected the amounts of some metabolites produced.