首页> 外文期刊>The Annals of Occupational Hygiene >EXPOSURE ASSESSMENT OF AIRBORNE MICROORGANISMS BY FLUORESCENCE MICROSCOPY AND IMAGE PROCESSING
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EXPOSURE ASSESSMENT OF AIRBORNE MICROORGANISMS BY FLUORESCENCE MICROSCOPY AND IMAGE PROCESSING

机译:荧光显微镜和图像处理技术对空气中微生物的暴露评估

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The p ose of this study was to improve the exposure assessment of airborne microorganisms by means of image processing of fluorescence microscopy images. This technique reduces the analysis time and also offers the opportunity to measure the size distribution of the microorganisms. We developed and implemented an automatic focusing procedure in order to count and size evaluate the microoganisms in the sample. However, automatic focusing was not possible if there were any impurities such as larger particles present. Therefore, manual focusing of the microscope had to be applied in connection with automatic counting and size evaluation when assessing the exposure of workers handling materials containing microorganisms, for example.
机译:这项研究的目的是通过荧光显微镜图像的图像处理来改善空气中微生物的暴露评估。该技术减少了分析时间,并且还提供了测量微生物大小分布的机会。我们开发并实施了自动聚焦程序,以便对样品中的微生物进行计数和大小评估。但是,如果存在任何杂质(例如较大的颗粒),则无法自动聚焦。因此,例如,当评估处理含微生物材料的工人的暴露时,必须结合自动计数和尺寸评估应用显微镜的手动聚焦。

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