首页> 外文期刊>Analytical Chemistry >VISIBLE DIODE LASER-INDUCED FLUORESCENCE DETECTION IN LIQUID CHROMATOGRAPHY AFTER PRECOLUMN DERIVATIZATION OF AMINES
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VISIBLE DIODE LASER-INDUCED FLUORESCENCE DETECTION IN LIQUID CHROMATOGRAPHY AFTER PRECOLUMN DERIVATIZATION OF AMINES

机译:胺柱前衍生化后液相色谱中可见二极管激光诱导的荧光检测

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摘要

To fully exploit the attractive visible diode laser-induced fluorescence (LIF) detection technique in column liquid chromatography (LC), covalent labeling procedures need to be developed which are compatible with near-infrared fluorescence. For this purpose, several red-absorbing labels containing a single succinimidyl ester functionality were synthesized and used for the derivatization of primary and secondary amines. Oxazines/thiazines, squaraines, and dicarbocyanines were examined as red-absorbing fluorophores. The quality of the LC-diode LIF system for the analytes concerned is illustrated by the concentration detection limit of the labeled n-octylamine (a test compound), which was as low as 2 x 10(-12) M. As expected in view of the limited reactivity of the succinimidyl ester group, the analyte concentrations required to obtain quantitative reaction with the label were equal to or higher than 2 x 10(-8) M. To show the applicability of the method to biological samples, urine was spiked with 5 x 10(-7) M. 1-adamantanamine, extracted, derivatized with a dicarbocyanine-based label, and analyzed by LC-diode LIF.
机译:为了在柱液相色谱(LC)中充分利用有吸引力的可见二极管激光诱导的荧光(LIF)检测技术,需要开发与近红外荧光兼容的共价标记程序。为此目的,合成了几种包含单个琥珀酰亚胺基酯官能团的吸收红的标记,并将其用于伯胺和仲胺的衍生化。恶嗪/噻嗪,方菜和二碳菁被视为吸收红光的荧光团。标记的正辛胺(一种测试化合物)的浓度检测极限可说明有关分析物的LC二极管LIF系统的质量低至2 x 10(-12)M。丁二酰亚胺酯基团的有限反应性中,与标记物进行定量反应所需的分析物浓度等于或高于2 x 10(-8)M。为表明该方法对生物样品的适用性,加标了尿液用5 x 10(-7)M. 1-金刚烷胺萃取,用基于二碳花青的标记衍生化,并通过LC二极管LIF分析。

著录项

  • 来源
    《Analytical Chemistry》 |1995年第10期|p.1742-1748|共7页
  • 作者单位

    Mank AJG, FREE UNIV AMSTERDAM, DEPT GEN & ANALYT CHEM, DE BOELELAAN 1083, 1081 HV AMSTERDAM, NETHERLANDS;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分析化学;
  • 关键词

    Dyes; Amantadine; Urine;

    机译:染料;金刚烷胺;尿液;
  • 入库时间 2022-08-18 01:05:39

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