首页> 外文期刊>Analytical Chemistry >APPLICATIONS OF WIRED PEROXIDASE ELECTRODES FOR PEROXIDE DETERMINATION IN LIQUID CHROMATOGRAPHY COUPLED TO OXIDASE IMMOBILIZED ENZYME REACTORS
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APPLICATIONS OF WIRED PEROXIDASE ELECTRODES FOR PEROXIDE DETERMINATION IN LIQUID CHROMATOGRAPHY COUPLED TO OXIDASE IMMOBILIZED ENZYME REACTORS

机译:有线过氧化物酶电极在过氧化物测定中的应用-液相色谱法与氧化酶固定化酶反应器联用

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An osmium poly(vinylpyridine) redox polymer ''wired'' horseradish peroxidase electrode has been used to detect H2O2 for the determination of glucose, lactate, and acetylcholine/choline with liquid chromatography (LC) and postcolumn immobilized enzyme reactors (IMERs). The redox polymer film containing peroxidase is coated on the surface of a glassy carbon electrode and operated at +100 mV vs Ag/AgCl for the reduction of H2O2. Compared with a conventional platinum electrode oxidizing H2O2 at +500 mV vs Ag/AgCl, the peroxidase cathode exhibits a 2-10-fold improvement in sensitivity and detection limit. The enzyme electrode also shows better operational stability than the Pt electrode. When the enzyme electrode is coupled to LC oxidase IMER systems, the initial stabilization of the background current is significantly faster than that for the conventional Pt electrode. The enzyme electrode has been used to determine glucose and lactate concentrations in rat subcutaneous microdialysates. After 8 days of continuous injection of the dialysate samples, the enzyme electrode sensitivity dropped by only 5%.
机译:liquid(乙烯基吡啶)氧化还原聚合物``有线''辣根过氧化物酶电极已用于通过液相色谱法(LC)和柱后固定化酶反应器(IMER)测定葡萄糖,乳酸和乙酰胆碱/胆碱中的过氧化氢。将含过氧化物酶的氧化还原聚合物薄膜涂覆在玻璃碳电极的表面,并在相对于Ag / AgCl的+100 mV电压下操作以减少H2O2。与在Ag / AgCl相比+500 mV的条件下将H2O2氧化的常规铂电极相比,过氧化物酶阴极的灵敏度和检测限提高了2-10倍。酶电极也显示出比Pt电极更好的操作稳定性。当酶电极与LC氧化酶IMER系统偶联时,背景电流的初始稳定度明显快于常规Pt电极。酶电极已用于确定大鼠皮下微量透析液中的葡萄糖和乳酸浓度。连续注入透析液样品8天后,酶电极灵敏度仅下降了5%。

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