Multiplexed Bead-Based Mesofluidic System for Gene Diagnosis and Genotyping

摘要

We have developed a novel multiplexed bead-based mesofluidicnsystem (MBMS) based on the specific recognitionnevents on the surface of a series of microbeadsn(diameter 250 μm) arranged in polydimethylsiloxanen(PDMS) microchannels (diameter 300 μm) with thenpredetermined order and assembled an apparatus implementingnautomatically the high-throughput bead-basednassay and further demonstrated its feasibility and flexibilitynof gene diagnosis and genotyping, such as u0001-thalassemianmutation detection and HLA-DQA genotyping. Thenapparatus, consisting of bead-based mesofluidic PDMSnchip, liquid-processing module, and fluorescence detectionnmodule, can integrate the procedure of automatedsampling,nhybridization reactions, washing, and in situnfluorescence detection. The results revealed that MBMSnis fast, has high sensitivity, and can be automated to carrynout parallel and multiplexed genotyping and has thenpotential to open up new routes to flexible, high-throughputnapproaches for bioanalysis.