Indium−Tin Oxide Coated Microfabricated Device for the Injection of a Single Cell into a Fused Silica Capillary for Chemical Cytometry

摘要

A microfabricated device is described for the capture andninjection of a single mammalian cell into a fused silicancapillary for subsequent analysis by chemical cytometry.nThe device consists of a 500 μm diameter well made fromnpolydimethylsiloxane on an indium-tin oxide coatednmicroscope slide. The bottom of the well contains a 2 μmnhigh aperture, which was designed to block passage ofncells. A cellular suspension was allowed to settle on thendevice, and aspiration through the aperture was used tontrap a single NG-108 cell. Untrapped cells were washednfrom the device, and a 150 μm outer diameter and 50nμm inner diameter capillary was placed in the well. Toninject a cell, voltage was applied to the indium-tin oxidenwhile simultaneously applying vacuum at the distal endnof the capillary.