Ultrathroughput Multiple Reaction Monitoring Mass Spectrometry

摘要

A novel transformation of the multiplexing potential to thenthroughput potential of multiple reaction monitoring massnspectrometry is presented for targeted quantitation ofnproteins. Herein, this method is termed as ultrathroughputnmultiple reaction monitoring (UMRM) mass spectrometry.nIt integrates the use of stable isotope dilutionmultiplenreaction monitoring mass spectrometry andnpeptide derivatization with inexpensive and commercialnchemicals. One-experiment quantitation of a commonnsignature peptide in 25 different samples demonstratesnproof-of-concept for the unprecedented throughput potentialnof the UMRM technology.