Rapid LC-MS Drug Metabolite Profiling Using Microsomal Enzyme Bioreactors in a Parallel Processing Format

摘要

There were three unfortunate errors which occurrednwhile writing this article. The first is the labeling ofn5-hydroxydiclofenac (5OH-DCF) as 5′-hydroxydiclofenacnthroughout the whole article (Figure 1, Scheme 2, andnin several parts of the text). The hydroxylation ofndiclofenac by Cyt P450 3A4 leads to 5-hydroxydiclofenacnand not 5′-hydroxydiclofenac. The second error occurrednin Scheme 2a, where we have mistakenly labeled CytnP450 2C8 instead of Cyt P450 2C9 as the enzymenresponsible for the metabolism of diclofenac into 4′-nhydroxydiclofenac (not 4-hydroxydiclofenac (4-OH-nDCF)). Cyt P450 2C9 is the enzyme responsible for thenmetabolism of diclofenac into 4′-hydroxydiclofenac (4′-nOH-DCF). The third and final error is in the interchang-ning of ref 6 with ref 7. Reference 6 should be Chang,nM.; Kim, E. J.; El-Shourbagy, T. A. Rapid Commun. MassnSpectrom. 2007, 21,64 72. Reference 7 should benCarlson, T. J.; Fisher, M. B. Comb. Chem. High Through-nput Screening 2008, 11, 258 264.