Intraalveloar expression of tumor necrosis factor-α gene during conventional and high-frequency ventilation

摘要

The effects of conventional mechanical ventilation (CMV) and high-frequency oscillatory ventilation (HFO) on intraalveolar expression of the tumor necrosis factor-α (TNF-α) gene were studied in surfac- tant-depleted rabbits. After lung lavage with saline, 13 rabbits were administered either CMV (n = 6) or HFO ( n = 7) for 1 h at an Fl_02 of 1.0 and a mean airway pressure of 13 cm H_2O. Lung lavage was then repeated. The rebbits' RNA was extracted from the lavage cells, and mRNA for TNF-α was quan- titated by reverse-transcription polymerase chain reaction using glyceraldehyde 3-phosphate dehy- drogenase (GAPDH) as an internal standard.