Effects of inhaled nitric oxide or inhibition of endogenous nitric oxide formation on hyperoxic lung injury

摘要

Nitric oxide (NO) may either protect against or contribute to oxidant-induced lung injury. In this study, we sought to determine whether either inhaled NO in concentration of 10 and 100 parts per million (ppm) or inhibition of endogenous NO formation with L-NG nitroarginine methyl ester (L-NAME) or aminoguanidine alters the extent of lung injury in rats breathing 100/100 O_2. Lung thiobarbituric acid reactive substances (TBARS), wet to dry lung weight ratio (Q_wQ_D), vascular and epithelial permeability (assessed by simultaneous intravenous administration of ~131l-labeled albumin and intraalveolar instillation of ~125l-labeled albumin), alveolar liquid clearance (evaluated based on the increase in alveolar protein concentration),and lung liquid clearance (gravimetric method) were determined after 40 h exposure to either 100/100or 21/100 O_2.