Effect of Glutamine Supplementation on Abilities of Antioxidative and γ-glutamyl Transpeptidase Activities in Liver and Intestine of Piglets Fed Diets Containing Raw Soybean

摘要

Eighteen male piglets weaned at 28 days age were randomly assigned to one of three treatments (1% glutamate, 1% and 2% glutamine supplementation). The basal diet contained 5% raw soybean. The diets were calculated to be isonitrogenous and isoenergetic. The level of plasma glutahione (GSH) increased markedly in piglets fed glutamine, and the response was related to dose. In treatments Ⅰ and Ⅱ, the levels of plasma GSH were significantly higher than that in the control at the 35 days age (P<0.05). The level of plasma GSH in treatment Ⅱ was significantly higher than that in the control at 42 days age (P<0.05). At 49 days age, there was no significantly difference of the level of GSH in plasma, liver, spleen, intestine and mes-enteric lymph node. The level of superoxide dismutase (SOD) in liver and spleen was higher than that of the control, however, the difference wasn't significant. Dietary glutamine supplementation decreased γ-glutamyl transpeptidase (γ-GT) activities in liver. The activities of γ-GT of liver protein in treatment Ⅱ were significantly lower than that in the control (P<0.05). The activities of γ-GT of duodenum in treatments Ⅰ and Ⅱ were also significantly lower than that in the control (P<0.05). But there were no significant differences of the activities of γ-GT in jejunum and ileum. The results showed that dietary glutamine supplementation increased the level of plasma GSH, and decreased γ-GT activities.