Optimization of Inactivation Conditions of High Hydrostatic Pressure Using Response Surface Methodology

摘要

Response surface methodology (RSM) was employed in the present work and a second order quadratic equation for high hydrostatic pressure (HHP) inactivation was built. The adequacy of the model equation for predicting the optimum response values was verified effectively by the validation data. Effects of temperature, pressure, and pressure holding time on HHP inactivation of Escherichia coli ATCC 8739 were explored. By analyzing the response surface plots and their corresponding contour plots as well as solving the quadratic equation, the optimum process parameters for inactivation E. coli of six log cycles were obtained as: temperature 32.2℃, pressure 346.4 MPa, and pressure holding time 12.6 min.