In Situ, Label-Free DNA Detection Using Organic Transistor Sensors

摘要

Rapid and highly sensitive PNA-DNA hybridization assays have attracted enormous attention for a wide variety of applications ranging from genotyping to molecular diagnosis. Conventional optical detection systems based on microarrays and real-time PCR involve expensive detection protocols, typically requiring a fluorescent dye and optical sources/detectors; however, this method has become the standard technique for quantifying the extent of hybridization between surface immobilized probes and fluorophore-labeled DNA targets.