Antisense Ca~2+ -calmodulin dependent protein kinase oligonu- cleotide inhibits bFGF-induced proliferation of rat vascular smooth muscle cells

摘要

PD98059 inhibited bFGF- induced phosphorylation of CCDPK and DNA synthesis. Antisense CCDPK ODN 0.2-0.8 μmol.L~-1 reduced Both p44- and p42-CCDPK expression and phosphoryla- Tion of CCDPK in a concentration-dependent manner and DNA synthesis induced by bFGF. bFGF-stimulated rat VSMC proliferation is medi- ated by CCDPK. The antisense CCDPK ODN can inhib- it bFGF-induced VSMC proliferation through down-regu- lating p44- and p42-CCDPK level.