Effect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts

摘要

Aim: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts. Methods: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed. Results: Daidzin, genistin, and glycitin 1 x 10~(-8), 5 x 10~(-7), 1 x 10~(-6), 5 x 10~(-6), and 1 x 10~(-5) mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5 x l0~(-7) mol/L and genistin 1 x 10~(-6) mol/L promoted the osteogenesis of MSC. Genistin 1 x 10~(-8), 5 x 10~(-7), 1 x 10~(-6), 5 x 10~(-6), and 1 x 10~(-5) mol/L and glycitin 1 x 10~(-8), 1 x 10~(-6), and 1 x 10~(-5) mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1 x 10~(-8), 5 x 10~(-7), 1 x 10~(-6), 5 x 10~(-6), and 1 x 10~(-5) mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. Conclusions: Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteo-blast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.