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VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses

机译:VAPA是一种创新的“病毒获取表型分析”方法,为植物病毒的载体传播研究开辟了新视野

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摘要

Host-to-host transmission—a key step in plant virus infection cycles—is ensured predominantly by vectors, especially aphids and related insects. A deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strategies, because any newly discovered molecular or cellular process is a potential target for hampering viral spread within host populations. With this aim in mind, an aphid membrane-feeding assay was developed where aphids transmitted two non-circulative viruses [cauliflower mosaic virus (CaMV) and turnip mosaic virus] from infected protoplasts. In this assay, virus acquisition occurs exclusively from living cells. Most interestingly, we also show that CaMV is less efficiently transmitted by aphids in the presence of oryzalin—a microtubule-depolymerising drug. The example presented here demonstrates that our technically simple “virus-acquisition phenotyping assay” (VAPA) provides a first opportunity to implement correlative studies relating the physiological state of infected plant cells to vector-transmission efficiency.
机译:宿主之间的传播是植物病毒感染周期中的关键步骤,主要通过媒介,尤其是蚜虫和相关昆虫来确保。对病毒获取机制(对载体传播至关重要)的更深入了解可能有助于设计未来的病毒控制策略,因为任何新发现的分子或细胞过程都是阻碍病毒在宿主群体中传播的潜在目标。出于这个目的,开发了一种蚜虫膜饲喂测定法,其中蚜虫传播了两种非循环病毒[花椰菜花叶病毒(CaMV)和芜菁花叶病毒],它们来自被感染的原生质体。在该测定中,病毒获取仅发生于活细胞。最有趣的是,我们还表明,在存在谷胱甘肽(一种微管解聚药物)的情况下,蚜虫传播CaMV的效率较低。此处提供的示例表明,我们技术上简单的“病毒获取表型分析”(VAPA)为实施相关研究提供了第一个机会,该研究将受感染植物细胞的生理状态与载体传播效率相关联。

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