Physiological levels of the PTEN-PI3K-AKT axis activity are required for maintenance of Burkitt lymphoma

摘要

Low PI3K-AKT activity in BL in comparison with GCB-DLBCL cell lines and tonsillar GC DZ B cells. , Expression and phosphorylation status AKT in BL and GCB-DLBCL cell lines was analysed by immunoblot. TUBB served as loading control. A representative of two independent experiments is shown. Short exposure. Long exposure. Immunoblots of pAKT and total AKT in three FACS-sorted tonsillar GC DZ B cells as well as two BL cell lines with lowest and highest pAKT expression levels. Expression and phosphorylation status of AKT in FACS-sorted tonsillar GC DZ B cells (sample #2 as shown in ) compared with BL and GCB-DLBCL cell lines with lowest and highest pAKT levels was analysed by immunoblot