首页> 美国卫生研究院文献>The Journal of Experimental Medicine >Generation of interleukin 4 (IL-4)-producing cells in vivo and in vitro: IL-2 and IL-4 are required for in vitro generation of IL-4- producing cells
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Generation of interleukin 4 (IL-4)-producing cells in vivo and in vitro: IL-2 and IL-4 are required for in vitro generation of IL-4- producing cells

机译:体内和体外产生白介素4(IL-4)的细胞的产生:产生IL-4的细胞的体外产生需要IL-2和IL-4

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摘要

T cell populations derived from naive mice produce very small amounts of interleukin 4 (IL-4) in response to stimulation on anti-CD3-coated dishes. IL-4 production by such cells is mainly found among large- and intermediate-sized T cells and is dependent upon IL-2. Injection of anti-IgD into mice, a stimulus that leads to striking increases in serum levels of IgG1 and IgE, causes a striking increase in the IL-4- producing capacity of T cells. This increase is first observed 4 d after injection of anti-IgD. IL-4 production by T cells from anti-IgD- injected donors is mainly found among large- and intermediate-sized T cells. Small, dense T cells are poor producers of IL-4. The capacity of T cells from anti-IgD-injected donors to produce IL-4 is enhanced by addition of IL-2 and is largely, but not completely, inhibited by neutralization of in situ produced IL-2. These results indicate that the control of IL-4 production in T cells from naive and anti-IgD- injected donors is similar. However, it is possible that a portion of the IL-4-producing activity of T cells from activated donors is IL-2 independent. Although small T cells from naive donors have a very limited capacity to produce IL-4 in response to stimulation with anti- CD3, even in the presence of added IL-2, they can give rise to IL-4- producing cells upon in vitro culture on plates coated with anti-CD3 if both IL-2 and IL-4 are added. This leads to the appearance of IL-4- producing cells within 2 d. When analyzed after 5 d of culture by harvesting and re-exposure to anti-CD3-coated culture wells and IL-2, these cells have increased their IL-4-producing capacity by approximately 100-fold. The development of IL-4-producing cells in response to anti-CD3, IL-2, and IL-4 is not inhibited by interferon gamma (IFN-gamma), nor does IFN-gamma diminish IL-4 production by these cells upon challenge with anti-CD3 plus IL-2.
机译:源自幼稚小鼠的T细胞群体响应于抗CD3涂层培养皿上的刺激而产生非常少量的白介素4(IL-4)。此类细胞产生的IL-4主要在大中型T细胞中发现,并且依赖于IL-2。向小鼠注射抗IgD的刺激会导致IgG1和IgE的血清水平显着增加,从而引起T细胞产生IL-4的能力显着增加。注射抗IgD后4天首先观察到这种增加。 T细胞从抗IgD注射的供体产生的IL-4主要在大中型T细胞中发现。小而致密的T细胞是IL-4的不良产生者。通过添加IL-2可以增强抗IgD注射供体的T细胞产生IL-4的能力,并且通过中和原位产生的IL-2可以很大程度上(但不完全)抑制T细胞的能力。这些结果表明,来自天真和抗IgD注射的供体的T细胞中IL-4产生的控制是相似的。但是,来自活化供体的T细胞产生IL-4的部分活性可能独立于IL-2。尽管来自幼稚供体的小T细胞响应抗CD3刺激产生IL-4的能力非常有限,即使存在添加的IL-2,它们也可以在体外产生IL-4的细胞如果同时加入IL-2和IL-4,则在涂有抗CD3的平板上进行培养。这导致在2天内出现产生IL-4的细胞。在培养5天后通过收获并重新暴露于抗CD3包被的培养孔和IL-2进行分析时,这些细胞的IL-4产生能力提高了约100倍。响应抗CD3,IL-2和IL-4的IL-4产生细胞的发育不受干扰素γ(IFN-γ)的抑制,IFN-γ也不会减少这些细胞在注射后产生的IL-4。用抗CD3加IL-2攻击。

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