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Effects of Temperature on the Characteristics of Nitrogen Removal and Microbial Community in Post Solid-Phase Denitrification Biofilter Process

机译:固相后反硝化生物滤池工艺中温度对氮去除特征和微生物群落的影响

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摘要

In order to solve the problems of high energy consumption, complex process and low nitrogen removal efficiency in the currently available low carbon source wastewater treatment processes, a novel coagulation sedimentation/post-solid-phase denitrification biofilter process (CS-BAF-SPDB) was proposed. The effect of temperature on the nitrogen removal performance of BAF-SPDB was intensively studied, and the mechanism of the effect of temperature on nitrogen removal performance was analyzed from the perspective of microbial community structure by using the polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that, to realize favorable nitrifying and denitrifying performance simultaneously in the BAF-SPDB unit, the operation temperature should be set above 18 °C. In addition, the influence of the macro operation parameters on the performance of the BAF and SPDB has a direct relationship with the dynamic changes of the micro microbial community. The influence of temperature on nitrification performance in BAF was mainly embodied in the change of composition, amount and activity of ammonia oxidizing bacteria and nitrite oxidizing bacteria , while that on denitrification performance in SPDB is mainly embodied in the change of composition and amount of solid carbon substrate degrading denitrifying bacteria sp., and heterotrophic denitrifying bacteria , , and .
机译:为了解决现有低碳源废水处理工艺中能耗高,工艺复杂,脱氮效率低的问题,提出了一种新型的混凝沉淀/固相后反硝化生物滤池工艺(CS-BAF-SPDB)。建议。深入研究了温度对BAF-SPDB脱氮性能的影响,并通过聚合酶链反应变性梯度凝胶电泳(PCR)从微生物群落结构的角度分析了温度对脱氮性能的影响机理。 -DGGE)。结果表明,为了同时在BAF-SPDB装置中实现良好的硝化和反硝化性能,操作温度应设置为18°C以上。此外,宏操作参数对BAF和SPDB性能的影响与微生物群落的动态变化有直接关系。温度对BAF中硝化性能的影响主要体现在氨氧化细菌和亚硝酸盐氧化细菌的组成,数量和活性的变化,而对SPDB中反硝化性能的影响主要体现在固体碳的组成和数量的变化。底物降解反硝化细菌sp。和异养反硝化细菌sp和。

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