In untreated cells of the marine pseudomonad studied here, alkaline phosphatase was found to be located in the periplasmic space, at the cell surface, and in the medium into which it had been shed during growth. Washing in 0.5 M NaCl, which removed the loosely bound outer layer, caused a shift of periplasmic enzyme to the outer aspect of the double-track layer and released some of the cell surface-associated enzyme. When the double-track layer of the cell wall was partially deranged, large amounts of this cell wall-associated enzyme were released, and, when the double-track was removed from the cells to produce mureinoplasts, alkaline phosphatase was released into the menstruum. There was no significant association of the enzyme with the peptidoglycan layer of the cell wall, which is the outermost structure of the mureinoplast, and no association of the enzyme with the cytoplasmic membrane of these modified cells. This study has shown that alkaline phosphatase is specifically associated with the outer layers of the cell walls of cells of this organism and is retained within the cell wall by virtue of this association.
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机译：在这里研究的未经处理的海洋假单胞菌细胞中，发现碱性磷酸酶位于周质空间，细胞表面以及在生长过程中掉入的培养基中。用0.5 M NaCl洗涤，除去了松散结合的外层，导致周质酶转移到双迹层的外表面，并释放了一些与细胞表面相关的酶。当细胞壁的双迹层部分地错开时，大量的这种与细胞壁相关的酶被释放，并且当从细胞中去除双迹以产生质膜体时，碱性磷酸酶被释放到月经中。所述酶与细胞壁的肽聚糖层没有明显的关联，所述肽聚糖层是mureinoplast的最外层结构，并且所述酶与这些修饰的细胞的细胞质膜也没有关联。这项研究表明，碱性磷酸酶与该生物体细胞壁的外层特异性结合，并通过这种结合而保留在细胞壁内。
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