sai-1 Mutation in Saccharomyces cerevisiae: Characteristics of Inhibition by S-Adenosylmethionine and S-Adenosylhomocysteine and Protection by Methionine

摘要

The relationship of methionine to the inhibition caused by S-adenosylmethionine and S-adenosylhomocysteine in strains containing the sai-1 mutation has been investigated and shown to affect indirectly the survival of the mutants. The ability of the mutants to take up both inhibitors is similar to that of the wild-type cells. The mutant also retains the ability to hydrolyze S-adenosylhomocysteine and incorporate the hydrolytic products into the various cellular fractions. Maximal inhibition of the sai-1 mutants occurs at an extracellular concentration of 0.005 mmS-adenosylmethionine and 0.025 to 0.05 mmS-adenosylhomocysteine when the cellular concentration is 0.05 mg (dry weight) per ml. The results suggest that the sai-1 mutation affects reaction(s) either not associated with methionine biosynthesis, or methionine synthesis and at least one other critical cellular function.