Egg cylinder development during in vitro extended embryo culture predicts the post transfer developmental potential of mouse blastocysts

摘要

a A representative immunofluorescence image of blastocyst cell number and allocation. Embryos were stained with SOX2 (red) for ICM and CDX2 (green) for TE. Scale bar, 100 μM. b A representative image of E 3.5 expanded embryos of similar quality prior to zona removal and embryo extended culture. Scale bar, 100 μM. c Representative 3D confocal images, images for egg cylinder volume analyses, epiblast cell number analysis, and TE outgrowth area for IVM (top row), IVF (center row), and VIVO (bottom row) embryos. Embryos were stained with DAPI for nuclei (blue), rhodamine phalloidin for F-actin (red), and antibody against POU5F1 (green). Scale bars, 100 μM