Gamete binning: chromosome-level and haplotype-resolved genome assembly enabled by high-throughput single-cell sequencing of gamete genomes

摘要

Overview of gamete binning. (a) Extraction of gamete nuclei. (b) Single-cell genome sequencing of haploid gametes and haplotype phasing. (c) Genetic map construction based on the recombination patterns in the gamete genomes. (d) Long-read sequencing of somatic material. (e) Separation of long reads based on genetic linkage groups using phased alleles. (f) Independent assembly of each haplotype of each linkage group. (g) Scaffolding assemblies to chromosome-level using the gamete-derived genetic map